This is an internal version that might contain incomplete information.

Gene Technology, 3 credits

Genteknik, 3 hp

TFKE38

Course starting semester

The course is disused.

Main field of study

Biotechnology Chemical Biology

Course level

First cycle

Course type

Programme course

Examiner

Lars-Göran Mårtensson

Director of studies or equivalent

Magdalena Svensson

Education components

Preliminary scheduled hours: 42 h
Recommended self-study hours: 38 h

Available for exchange students

Yes

Possible deviation from course syllabus/programme syllabus

ECV = Elective / Compulsory / Voluntary

Course offered for		Semester	Period	Timetable module	Language	Campus	ECV
6KKEB	Chemical Biology	5 (Autumn 2017)	1	-	English	Linköping, Valla	C
6CKEB	Chemical Biology, M Sc in Engineering	5 (Autumn 2017)	1	-	English	Linköping, Valla	C
6MPRO	Protein Science, Master's Programme (External)	1 (Autumn 2017)	1	-	English	Linköping, Valla	C

Main field of study

Biotechnology, Chemical Biology

Course level

First cycle

Advancement level

G2X

Course offered for

Protein Science, Master's Programme
Chemical Biology, M Sc in Engineering
Chemical Biology

Entry requirements

Note: Admission requirements for non-programme students usually also include admission requirements for the programme and threshold requirements for progression within the programme, or corresponding.

Prerequisites

Biochemistry

Intended learning outcomes

The course will give fundamental knowledge and experimental skills in the following areas of Molecular Biology: Site-specific mutagenesis, gene cloning and PCR After studies well learned the student will have proficiency to:

Understand basic laboratory techniques in molecular biology techniques to perform site-directed mutagenesis, amplification of DNA fragments using PCR and cloning of DNA fragments
Describe different types of vectors for cloning and expression of eukaryotic genes in a prokaryotic system
Theoretical knowledge of DNA sequencing
Theoretical and practical knowledge of techniques for purification of plasmid DNA
Describe the function and applications of different enzymes involved in gene cloning experiments

Course content

Purifications of DNA, manipulation of DNA, cloning of genes into E.coli vectors, control of gene expression, differences in prokaryotic and eukaryotic genes, mutation analysis, DNA-sequencing.

Teaching and working methods

The theory is mainly presented in lectures. Solving of problems, discussions of theoretical and practical aspects of experiments in the laboratory are performed in lessons. Theoretical and practical aspects are illustrated in the laboratory course. The results of the experimental work should be presented and discussed in written reports. Laboration course in site-directed mutagenesis.

Examination

LAB1	Laboratory work	1 credits	U, G
TEN2	Written examination	2 credits	U, 3, 4, 5

Grades

Four-grade scale, LiU, U, 3, 4, 5

Other information

Supplementary courses: Protein chemistry.

Department

Institutionen för fysik, kemi och biologi

Director of Studies or equivalent

Magdalena Svensson

Examiner

Lars-Göran Mårtensson

Education components

Preliminary scheduled hours: 42 h
Recommended self-study hours: 38 h

Course literature

T.A. Brown: Gene Cloning and DNA analysis, 6th edition.

Code	Name	Scope	Grading scale
LAB1	Laboratory work	1 credits	U, G
TEN2	Written examination	2 credits	U, 3, 4, 5

Regulations (apply to LiU in its entirety)

The university is a government agency whose operations are regulated by legislation and ordinances, which include the Higher Education Act and the Higher Education Ordinance. In addition to legislation and ordinances, operations are subject to several policy documents. The Linköping University rule book collects currently valid decisions of a regulatory nature taken by the university board, the vice-chancellor and faculty/department boards.

LiU’s rule book for education at first-cycle and second-cycle levels is available at http://styrdokument.liu.se/Regelsamling/Innehall/Utbildning_pa_grund-_och_avancerad_niva.

Student rights and obligations (login required)
Linköping University common rules and regulations (in Swedish)

Course literature is preliminary.

T.A. Brown: Gene Cloning and DNA analysis, 6th edition.

Download

Download course literature list as PDF

Note: The course matrix might contain more information in Swedish.

I = Introduce, U = Teach, A = Utilize

		U	A	Modules
1. DISCIPLINARY KNOWLEDGE AND REASONING
	1.1 Knowledge of underlying mathematics and science (G1X level)		X	TEN2
	1.2 Fundamental engineering knowledge (G1X level)		X	TEN2
	1.3 Further knowledge, methods, and tools in one or several subjects in engineering or natural science (G2X level)		X	TEN2
	1.4 Advanced knowledge, methods, and tools in one or several subjects in engineering or natural sciences (A1X level)
	1.5 Insight into current research and development work
2. PERSONAL AND PROFESSIONAL SKILLS AND ATTRIBUTES
	2.1 Analytical reasoning and problem solving		X	LAB1
	2.2 Experimentation, investigation, and knowledge discovery	X		LAB1
	2.3 System thinking		X
	2.4 Attitudes, thought, and learning		X	LAB1
	2.5 Ethics, equity, and other responsibilities		X	LAB1
3. INTERPERSONAL SKILLS: TEAMWORK AND COMMUNICATION
	3.1 Teamwork
	3.2 Communications		X	LAB1
	3.3 Communication in foreign languages		X
4. CONCEIVING, DESIGNING, IMPLEMENTING AND OPERATING SYSTEMS IN THE ENTERPRISE, SOCIETAL AND ENVIRONMENTAL CONTEXT
	4.1 External, societal, and environmental context
	4.2 Enterprise and business context
	4.3 Conceiving, system engineering and management
	4.4 Designing
	4.5 Implementing
	4.6 Operating
5. PLANNING, EXECUTION AND PRESENTATION OF RESEARCH DEVELOPMENT PROJECTS WITH RESPECT TO SCIENTIFIC AND SOCIETAL NEEDS AND REQUIREMENTS
	5.1 Societal conditions, including economic, social, and ecological aspects of sustainable development for knowledge development
	5.2 Economic conditions for knowledge development
	5.3 Identification of needs, structuring and planning of research or development projects
	5.4 Execution of research or development projects
	5.5 Presentation and evaluation of research or development projects

This tab contains public material from the course room in Lisam. The information published here is not legally binding, such material can be found under the other tabs on this page.

There are no files available for this course.