Biostructural Technologies, 6 credits

Biomolekylär strukturanalys, 6 hp

TFKE35

The course is disused.

Main field of study

Chemical Biology

Course level

Second cycle

Course type

Programme course

Examiner

Patrik Lundström

Director of studies or equivalent

Magdalena Svensson

Education components

Preliminary scheduled hours: 36 h
Recommended self-study hours: 124 h

Available for exchange students

Yes

Possible deviation from course syllabus/programme syllabus

ECV = Elective / Compulsory / Voluntary

Course offered for		Semester	Period	Timetable module	Language	Campus	ECV
6CKEB	Chemical Biology, M Sc in Engineering (Protein Science and Technology)	7 (Autumn 2017)	2	2	Swedish/English	Linköping, Valla	C
6MPRO	Protein Science, Master's Programme (External)	1 (Autumn 2017)	2	2	Swedish/English	Linköping, Valla	C
6MPRO	Protein Science, Master's Programme (Internal)	1 (Autumn 2017)	2	2	Swedish/English	Linköping, Valla	C

Main field of study

Chemical Biology

Course level

Second cycle

Advancement level

A1X

Course offered for

Protein Science, Master's Programme
Chemical Biology, M Sc in Engineering

Entry requirements

Note: Admission requirements for non-programme students usually also include admission requirements for the programme and threshold requirements for progression within the programme, or corresponding.

Prerequisites

Organic chemistry 2 or similar. Biochemistry 1 or similar.

Intended learning outcomes

The course aims at providing the basic theoretical framework for NMR spectroscopy and x-ray crystallography, which is the foundation for structural analysis of proteins and other biomolecules in organic chemistry and structural biology. After completed course, the student should be able to:

Master and explain important concepts, methods and theories in the determination of molecular structure and dynamics.
Illustrate and shed light on theoretical concepts with examples from biomolecular analysis.
Interpret, assess and evaluate experimental results.
Propose and motivate an optimal strategy for solving a given problem within structural biology or bioorganic chemistry.

Course content

The NMR part of this course comprises: The NMR phenomenon, resonance, chemical shift, spin-spin couplings (weak and strong), dipolar couplings, nuclear spin relaxation, especially the nuclear Overhauser effect. Dynamic NMR, chemical exchange. Two dimensional NMR spectroscopy, COSY, TOCSY, NOESY. Heteronuclear experiments, HSQC. Analysis of onedimensional and multidimensional spectra. Procedures for structural and dynamical characterization of proteins.

The crystallography part of this course comprises: protein crystallization, including concepts such as unit cell, asymmetrical unit, space group, Miller index, Bragg’s law and Evald sphere. Furthermore, the entire process of solving a protein structure starting from a protein crystal will be treated, including concepts such as resolution, electron density maps, R-factor, B-factor, heavy metal derivatization and molecular replacement.

Teaching and working methods

Lectures, seminars, demonstrations.

Examination

KTR1	Written test	1.5 credits	U, 3, 4, 5
TEN1	Written examination	4.5 credits	U, 3, 4, 5

Grades

Four-grade scale, LiU, U, 3, 4, 5

Department

Institutionen för fysik, kemi och biologi

Director of Studies or equivalent

Magdalena Svensson

Examiner

Patrik Lundström

Education components

Preliminary scheduled hours: 36 h
Recommended self-study hours: 124 h

Course literature

Additional literature

Books

Gale Rodes, (2000) Crystallography made Crystal Clear 2nd edition
P. Lundström, (2012) NMR Spectroscopy – Principles and Applications to Proteins.

Code	Name	Scope	Grading scale
KTR1	Written test	1.5 credits	U, 3, 4, 5
TEN1	Written examination	4.5 credits	U, 3, 4, 5

Regulations (apply to LiU in its entirety)

The university is a government agency whose operations are regulated by legislation and ordinances, which include the Higher Education Act and the Higher Education Ordinance. In addition to legislation and ordinances, operations are subject to several policy documents. The Linköping University rule book collects currently valid decisions of a regulatory nature taken by the university board, the vice-chancellor and faculty/department boards.

LiU’s rule book for education at first-cycle and second-cycle levels is available at http://styrdokument.liu.se/Regelsamling/Innehall/Utbildning_pa_grund-_och_avancerad_niva.

Student rights and obligations (login required)
Linköping University common rules and regulations (in Swedish)

Course literature is preliminary.

Additional literature

Books

Gale Rodes, (2000) Crystallography made Crystal Clear 2nd edition

P. Lundström, (2012) NMR Spectroscopy – Principles and Applications to Proteins.

Download

Download course literature list as PDF

Note: The course matrix might contain more information in Swedish.

I = Introduce, U = Teach, A = Utilize

		I	U	A	Modules
1. DISCIPLINARY KNOWLEDGE AND REASONING
	1.1 Knowledge of underlying mathematics and science (G1X level)		X	X	KTR1 TEN1
	1.2 Fundamental engineering knowledge (G1X level)		X	X	KTR1 TEN1
	1.3 Further knowledge, methods, and tools in one or several subjects in engineering or natural science (G2X level)	X	X	X	KTR1 TEN1
	1.4 Advanced knowledge, methods, and tools in one or several subjects in engineering or natural sciences (A1X level)
	1.5 Insight into current research and development work
2. PERSONAL AND PROFESSIONAL SKILLS AND ATTRIBUTES
	2.1 Analytical reasoning and problem solving		X	X	KTR1 TEN1
	2.2 Experimentation, investigation, and knowledge discovery		X	X
	2.3 System thinking
	2.4 Attitudes, thought, and learning		X	X
	2.5 Ethics, equity, and other responsibilities
3. INTERPERSONAL SKILLS: TEAMWORK AND COMMUNICATION
	3.1 Teamwork			X
	3.2 Communications
	3.3 Communication in foreign languages			X
4. CONCEIVING, DESIGNING, IMPLEMENTING AND OPERATING SYSTEMS IN THE ENTERPRISE, SOCIETAL AND ENVIRONMENTAL CONTEXT
	4.1 External, societal, and environmental context
	4.2 Enterprise and business context
	4.3 Conceiving, system engineering and management
	4.4 Designing
	4.5 Implementing
	4.6 Operating
5. PLANNING, EXECUTION AND PRESENTATION OF RESEARCH DEVELOPMENT PROJECTS WITH RESPECT TO SCIENTIFIC AND SOCIETAL NEEDS AND REQUIREMENTS
	5.1 Societal conditions, including economic, social, and ecological aspects of sustainable development for knowledge development
	5.2 Economic conditions for knowledge development
	5.3 Identification of needs, structuring and planning of research or development projects
	5.4 Execution of research or development projects
	5.5 Presentation and evaluation of research or development projects

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