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Preparation of Proteins, 3 credits
Proteinpreparation, 3 hp
TFKE54
Main field of study
Biology Chemical BiologyCourse level
Second cycleCourse type
Programme courseExaminer
Lars-Göran MårtenssonDirector of studies or equivalent
Magdalena SvenssonEducation components
Preliminary scheduled hours: 34 hRecommended self-study hours: 46 h
Available for exchange students
YesECV = Elective / Compulsory / Voluntary
| Course offered for | Semester | Period | Timetable module | Language | Campus | ECV | |
|---|---|---|---|---|---|---|---|
| 6KBIO | 6 (Spring 2017) | 1 | 3 | Swedish/English | Linköping, Valla | E | |
| 6KKEB | Chemical Biology | 6 (Spring 2017) | 1 | 3 | Swedish/English | Linköping, Valla | C |
| 6KKEM | Chemistry | 6 (Spring 2017) | 1 | 3 | Swedish/English | Linköping, Valla | E |
| 6MKOS | Organic Synthesis and Medicinal Chemistry, Master's programme | 2 (Spring 2017) | 1 | 3 | Swedish/English | Linköping, Valla | C |
| 6MPRO | Protein Science, Master's programme (External) | 2 (Spring 2017) | 1 | 3 | Swedish/English | Linköping, Valla | E |
Main field of study
Biology, Chemical BiologyCourse level
Second cycleAdvancement level
A1XCourse offered for
- Chemistry
- Protein Science, Master's programme
- Organic Synthesis and Medicinal Chemistry, Master's programme
- Chemical Biology
Entry requirements
Note: Admission requirements for non-programme students usually also include admission requirements for the programme and threshold requirements for progression within the programme, or corresponding.
Prerequisites
Protein ChemistryIntended learning outcomes
The objective of the course is to provide comprehensive knowledge in the following areas: Protein chemistry, expression of cloned proteins and methodologies for protein purification as well as experimental planning. After studies well learned the student will have proficiency to:
- Express and purify cloned proteins
- Adopt a reflecting, scientific attitude to theories and obtained experimental results.
Course content
During the course methods such as expression of cloned proteins, affinity chromatography and SDS-PAGE are used. Various spectroscopic techniques such as absorbance, fluorescence and circular dichroism are used.
Teaching and working methods
The course has a pronounced experimental focus and character. Work is done on genetically mutated human proteins cloned in bacteria. The origin of the mutated proteins can be proteins that have been mutated by the students in an earlier course in gene technology or can be provided by the research group. The protein variants are expressed in E.coli and purified by affinity chromatography. Purity and yield are determined. The students should then independently evaluate their experimental data. The project is reported as a report.
Examination
| PRA1 | Project work | 3 credits | U, G |
Grades are given as ’Fail’ or ’Pass’.
Grades
Two-grade scale, U, GDepartment
Institutionen för fysik, kemi och biologiDirector of Studies or equivalent
Magdalena SvenssonExaminer
Lars-Göran MårtenssonEducation components
Preliminary scheduled hours: 34 hRecommended self-study hours: 46 h
Course literature
Kompendium och vetenskapliga artiklar| Code | Name | Scope | Grading scale |
|---|---|---|---|
| PRA1 | Project work | 3 credits | U, G |
Grades are given as ’Fail’ or ’Pass’.
Regulations (apply to LiU in its entirety)
The university is a government agency whose operations are regulated by legislation and ordinances, which include the Higher Education Act and the Higher Education Ordinance. In addition to legislation and ordinances, operations are subject to several policy documents. The Linköping University rule book collects currently valid decisions of a regulatory nature taken by the university board, the vice-chancellor and faculty/department boards.
LiU’s rule book for education at first-cycle and second-cycle levels is available at http://styrdokument.liu.se/Regelsamling/Innehall/Utbildning_pa_grund-_och_avancerad_niva.
Course literature is preliminary.
Kompendium och vetenskapliga artiklar
Note: The course matrix might contain more information in Swedish.
I = Introduce, U = Teach, A = Utilize
| I | U | A | Modules | Comment | ||
|---|---|---|---|---|---|---|
| 1. DISCIPLINARY KNOWLEDGE AND REASONING | ||||||
| 1.1 Knowledge of underlying mathematics and science (G1X level) |
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X
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| 1.2 Fundamental engineering knowledge (G1X level) |
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X
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| 1.3 Further knowledge, methods, and tools in one or several subjects in engineering or natural science (G2X level) |
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X
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| 1.4 Advanced knowledge, methods, and tools in one or several subjects in engineering or natural sciences (A1X level) |
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| 1.5 Insight into current research and development work |
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| 2. PERSONAL AND PROFESSIONAL SKILLS AND ATTRIBUTES | ||||||
| 2.1 Analytical reasoning and problem solving |
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X
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| 2.2 Experimentation, investigation, and knowledge discovery |
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X
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| 2.3 System thinking |
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X
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| 2.4 Attitudes, thought, and learning |
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X
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| 2.5 Ethics, equity, and other responsibilities |
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X
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| 3. INTERPERSONAL SKILLS: TEAMWORK AND COMMUNICATION | ||||||
| 3.1 Teamwork |
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X
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| 3.2 Communications |
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X
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| 3.3 Communication in foreign languages |
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X
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| 4. CONCEIVING, DESIGNING, IMPLEMENTING AND OPERATING SYSTEMS IN THE ENTERPRISE, SOCIETAL AND ENVIRONMENTAL CONTEXT | ||||||
| 4.1 External, societal, and environmental context |
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| 4.2 Enterprise and business context |
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| 4.3 Conceiving, system engineering and management |
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| 4.4 Designing |
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| 4.5 Implementing |
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| 4.6 Operating |
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| 5. PLANNING, EXECUTION AND PRESENTATION OF RESEARCH DEVELOPMENT PROJECTS WITH RESPECT TO SCIENTIFIC AND SOCIETAL NEEDS AND REQUIREMENTS | ||||||
| 5.1 Societal conditions, including economic, social, and ecological aspects of sustainable development for knowledge development |
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| 5.2 Economic conditions for knowledge development |
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| 5.3 Identification of needs, structuring and planning of research or development projects |
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X
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| 5.4 Execution of research or development projects |
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X
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| 5.5 Presentation and evaluation of research or development projects |
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X
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