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Proteomics, 6 credits
Proteomik, 6 hp
TFKE57
Main field of study
Chemical BiologyCourse level
Second cycleCourse type
Programme courseExaminer
Karin EnanderDirector of studies or equivalent
Magdalena SvenssonEducation components
Preliminary scheduled hours: 36 hRecommended self-study hours: 124 h
Available for exchange students
YesECV = Elective / Compulsory / Voluntary
| Course offered for | Semester | Period | Timetable module | Language | Campus | ECV | |
|---|---|---|---|---|---|---|---|
| 6CKEB | Chemical Biology, M Sc in Engineering (Protein Science and Technology) | 7 (Autumn 2017) | 1 | 3 | English | Linköping, Valla | C |
| 6MPRO | Protein Science, Master's programme (External) | 3 (Autumn 2017) | 1 | 3 | English | Linköping, Valla | C |
| 6MPRO | Protein Science, Master's Programme (Internal) | 1 (Autumn 2017) | 1 | 3 | English | Linköping, Valla | C |
Main field of study
Chemical BiologyCourse level
Second cycleAdvancement level
A1XCourse offered for
- Protein Science, Master's Programme
- Chemical Biology, M Sc in Engineering
- Protein Science, Master's programme
Entry requirements
Note: Admission requirements for non-programme students usually also include admission requirements for the programme and threshold requirements for progression within the programme, or corresponding.
Prerequisites
Admission to advanced level studies in Chemical Biology. Protein chemistry, gene technology and biological measurements. Basic knowledge of analytical chemistry and bioinformatics is also recommended.Intended learning outcomes
The aim of the course is to provide a broad knowledge of methods and applications within proteomics. Many of the basic methodological principles (genetic, biochemical, analytical chemical and bioinformatic) have been introduced in earlier courses, but here the student learns how these principles are used within a currently very expansive research area: Global characterisation of the proteome with regard to protein abundance, function, regulation, interactions and localisation in different cell types. Transcriptomics is also discussed. To pass the course, the student should be able to
- describe the relevance and distinctive character of proteomics
- describe and discuss principles and applications of modern methods and strategies to analyse the transcriptome and the proteome
- identify proteins and peptides using mass spectra and genome databases
- summarise the contents of selected original proteomics literature, interpret its message and review it critically
Course content
Quantitative mRNA-analysis with DNA microarrays and other methods. Multi-dimensional protein separation methods (2DGE, 2DLC). Identification of cellular protein contents using enzymatic cleavage, MS- and MS/MS-analysis and genome databases. Protein quantification based on isotope labelling and MS. Analysis of post-translational modifications. Analysis of protein interactions using affinity chromatography, Y2H methodology and protein microarrays. Characterisation of changes of the cellular protein contents following changes in the environment, for e. g. identification of disease biomarkers. Metaproteomics. Human Proteome Atlas.
Teaching and working methods
The course consists of lectures, seminars, demonstrations, and two home assignments.
Examination
| UPG2 | Presentation | 1 credits | U, G |
| UPG1 | Hand-in assignments | 1 credits | U, G |
| TEN1 | Written examination | 4 credits | U, 3, 4, 5 |
Grades
Four-grade scale, LiU, U, 3, 4, 5Department
Institutionen för fysik, kemi och biologiDirector of Studies or equivalent
Magdalena SvenssonExaminer
Karin EnanderEducation components
Preliminary scheduled hours: 36 hRecommended self-study hours: 124 h
Course literature
Twyman, R. M.; ”Principles of Proteomics” 2nd edition. Originalartiklar.| Code | Name | Scope | Grading scale |
|---|---|---|---|
| UPG2 | Presentation | 1 credits | U, G |
| UPG1 | Hand-in assignments | 1 credits | U, G |
| TEN1 | Written examination | 4 credits | U, 3, 4, 5 |
Regulations (apply to LiU in its entirety)
The university is a government agency whose operations are regulated by legislation and ordinances, which include the Higher Education Act and the Higher Education Ordinance. In addition to legislation and ordinances, operations are subject to several policy documents. The Linköping University rule book collects currently valid decisions of a regulatory nature taken by the university board, the vice-chancellor and faculty/department boards.
LiU’s rule book for education at first-cycle and second-cycle levels is available at http://styrdokument.liu.se/Regelsamling/Innehall/Utbildning_pa_grund-_och_avancerad_niva.
Course literature is preliminary.
Twyman, R. M.; ”Principles of Proteomics” 2nd edition. Originalartiklar.
Note: The course matrix might contain more information in Swedish.
I = Introduce, U = Teach, A = Utilize
| I | U | A | Modules | Comment | ||
|---|---|---|---|---|---|---|
| 1. DISCIPLINARY KNOWLEDGE AND REASONING | ||||||
| 1.1 Knowledge of underlying mathematics and science (G1X level) |
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X
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| 1.2 Fundamental engineering knowledge (G1X level) |
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X
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| 1.3 Further knowledge, methods, and tools in one or several subjects in engineering or natural science (G2X level) |
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X
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TEN1
UPG1
UPG2
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| 1.4 Advanced knowledge, methods, and tools in one or several subjects in engineering or natural sciences (A1X level) |
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| 1.5 Insight into current research and development work |
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| 2. PERSONAL AND PROFESSIONAL SKILLS AND ATTRIBUTES | ||||||
| 2.1 Analytical reasoning and problem solving |
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X
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TEN1
UPG1
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| 2.2 Experimentation, investigation, and knowledge discovery |
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| 2.3 System thinking |
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| 2.4 Attitudes, thought, and learning |
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X
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X
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TEN1
UPG1
UPG2
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| 2.5 Ethics, equity, and other responsibilities |
X
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X
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UPG2
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| 3. INTERPERSONAL SKILLS: TEAMWORK AND COMMUNICATION | ||||||
| 3.1 Teamwork |
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X
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X
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UPG1
UPG2
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| 3.2 Communications |
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X
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X
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TEN1
UPG1
UPG2
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| 3.3 Communication in foreign languages |
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X
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X
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UPG2
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| 4. CONCEIVING, DESIGNING, IMPLEMENTING AND OPERATING SYSTEMS IN THE ENTERPRISE, SOCIETAL AND ENVIRONMENTAL CONTEXT | ||||||
| 4.1 External, societal, and environmental context |
X
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| 4.2 Enterprise and business context |
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| 4.3 Conceiving, system engineering and management |
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| 4.4 Designing |
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| 4.5 Implementing |
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| 4.6 Operating |
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| 5. PLANNING, EXECUTION AND PRESENTATION OF RESEARCH DEVELOPMENT PROJECTS WITH RESPECT TO SCIENTIFIC AND SOCIETAL NEEDS AND REQUIREMENTS | ||||||
| 5.1 Societal conditions, including economic, social, and ecological aspects of sustainable development for knowledge development |
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| 5.2 Economic conditions for knowledge development |
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| 5.3 Identification of needs, structuring and planning of research or development projects |
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| 5.4 Execution of research or development projects |
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| 5.5 Presentation and evaluation of research or development projects |
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