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Gene Technology, 3 credits
Genteknik, 3 hp
TFKE38
Main field of study
Biotechnology Chemical BiologyCourse level
First cycleCourse type
Programme courseExaminer
Lars-Göran MårtenssonDirector of studies or equivalent
Magdalena SvenssonEducation components
Preliminary scheduled hours: 42 hRecommended self-study hours: 38 h
Available for exchange students
YesECV = Elective / Compulsory / Voluntary
| Course offered for | Semester | Period | Timetable module | Language | Campus | ECV | |
|---|---|---|---|---|---|---|---|
| 6KKEB | Chemical Biology | 5 (Autumn 2017) | 1 | - | English | Linköping, Valla | C |
| 6CKEB | Chemical Biology, M Sc in Engineering | 5 (Autumn 2017) | 1 | - | English | Linköping, Valla | C |
| 6MPRO | Protein Science, Master's Programme (External) | 1 (Autumn 2017) | 1 | - | English | Linköping, Valla | C |
Main field of study
Biotechnology, Chemical BiologyCourse level
First cycleAdvancement level
G2XCourse offered for
- Protein Science, Master's Programme
- Chemical Biology, M Sc in Engineering
- Chemical Biology
Entry requirements
Note: Admission requirements for non-programme students usually also include admission requirements for the programme and threshold requirements for progression within the programme, or corresponding.
Prerequisites
BiochemistryIntended learning outcomes
The course will give fundamental knowledge and experimental skills in the following areas of Molecular Biology: Site-specific mutagenesis, gene cloning and PCR After studies well learned the student will have proficiency to:
- Understand basic laboratory techniques in molecular biology techniques to perform site-directed mutagenesis, amplification of DNA fragments using PCR and cloning of DNA fragments
- Describe different types of vectors for cloning and expression of eukaryotic genes in a prokaryotic system
- Theoretical knowledge of DNA sequencing
- Theoretical and practical knowledge of techniques for purification of plasmid DNA
- Describe the function and applications of different enzymes involved in gene cloning experiments
Course content
Purifications of DNA, manipulation of DNA, cloning of genes into E.coli vectors, control of gene expression, differences in prokaryotic and eukaryotic genes, mutation analysis, DNA-sequencing.
Teaching and working methods
The theory is mainly presented in lectures. Solving of problems, discussions of theoretical and practical aspects of experiments in the laboratory are performed in lessons. Theoretical and practical aspects are illustrated in the laboratory course. The results of the experimental work should be presented and discussed in written reports. Laboration course in site-directed mutagenesis.
Examination
| LAB1 | Laboratory work | 1 credits | U, G |
| TEN2 | Written examination | 2 credits | U, 3, 4, 5 |
Grades
Four-grade scale, LiU, U, 3, 4, 5Other information
Supplementary courses: Protein chemistry.
Department
Institutionen för fysik, kemi och biologiDirector of Studies or equivalent
Magdalena SvenssonExaminer
Lars-Göran MårtenssonEducation components
Preliminary scheduled hours: 42 hRecommended self-study hours: 38 h
Course literature
T.A. Brown: Gene Cloning and DNA analysis, 6th edition.| Code | Name | Scope | Grading scale |
|---|---|---|---|
| LAB1 | Laboratory work | 1 credits | U, G |
| TEN2 | Written examination | 2 credits | U, 3, 4, 5 |
Regulations (apply to LiU in its entirety)
The university is a government agency whose operations are regulated by legislation and ordinances, which include the Higher Education Act and the Higher Education Ordinance. In addition to legislation and ordinances, operations are subject to several policy documents. The Linköping University rule book collects currently valid decisions of a regulatory nature taken by the university board, the vice-chancellor and faculty/department boards.
LiU’s rule book for education at first-cycle and second-cycle levels is available at http://styrdokument.liu.se/Regelsamling/Innehall/Utbildning_pa_grund-_och_avancerad_niva.
Course literature is preliminary.
T.A. Brown: Gene Cloning and DNA analysis, 6th edition.
Note: The course matrix might contain more information in Swedish.
I = Introduce, U = Teach, A = Utilize
| I | U | A | Modules | Comment | ||
|---|---|---|---|---|---|---|
| 1. DISCIPLINARY KNOWLEDGE AND REASONING | ||||||
| 1.1 Knowledge of underlying mathematics and science (G1X level) |
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X
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TEN2
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| 1.2 Fundamental engineering knowledge (G1X level) |
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X
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TEN2
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| 1.3 Further knowledge, methods, and tools in one or several subjects in engineering or natural science (G2X level) |
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X
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TEN2
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| 1.4 Advanced knowledge, methods, and tools in one or several subjects in engineering or natural sciences (A1X level) |
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| 1.5 Insight into current research and development work |
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| 2. PERSONAL AND PROFESSIONAL SKILLS AND ATTRIBUTES | ||||||
| 2.1 Analytical reasoning and problem solving |
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X
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LAB1
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| 2.2 Experimentation, investigation, and knowledge discovery |
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X
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LAB1
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| 2.3 System thinking |
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X
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| 2.4 Attitudes, thought, and learning |
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X
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LAB1
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| 2.5 Ethics, equity, and other responsibilities |
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X
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LAB1
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| 3. INTERPERSONAL SKILLS: TEAMWORK AND COMMUNICATION | ||||||
| 3.1 Teamwork |
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| 3.2 Communications |
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X
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LAB1
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| 3.3 Communication in foreign languages |
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X
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| 4. CONCEIVING, DESIGNING, IMPLEMENTING AND OPERATING SYSTEMS IN THE ENTERPRISE, SOCIETAL AND ENVIRONMENTAL CONTEXT | ||||||
| 4.1 External, societal, and environmental context |
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| 4.2 Enterprise and business context |
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| 4.3 Conceiving, system engineering and management |
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| 4.4 Designing |
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| 4.5 Implementing |
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| 4.6 Operating |
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| 5. PLANNING, EXECUTION AND PRESENTATION OF RESEARCH DEVELOPMENT PROJECTS WITH RESPECT TO SCIENTIFIC AND SOCIETAL NEEDS AND REQUIREMENTS | ||||||
| 5.1 Societal conditions, including economic, social, and ecological aspects of sustainable development for knowledge development |
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| 5.2 Economic conditions for knowledge development |
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| 5.3 Identification of needs, structuring and planning of research or development projects |
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| 5.4 Execution of research or development projects |
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| 5.5 Presentation and evaluation of research or development projects |
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